Cytokine concentrations in plasma samples were evaluated using ProcartaPlexTM immunoassays (eBioscience, San Diego, CA, United States) according to the manufacturer’s specifications (Mayerhofer et al., 2017 (link)). Fluorescent signals were quantified with the Bio-Plex 200 multiplex suspension array system equipped with Luminex® xMAP® technology combined with the Bio-Plex 5.0 software (BioRad, Hercules, CA, United States).
Cytokines that were too low to be detected were excluded from further analysis. All cytokine concentrations were evaluated in duplicates. It should not go unmentioned at this point that IL-1β levels were analyzed in the blood plasma, but were too low to be detected throughout all groups and will not be displayed in the results part.
Corticosterone plasma levels were determined with a specific enzyme immunoassay kit (Assay Designs, Ann Arbor, MI, United States) with a sensitivity of 0.027 ng/ml as previously described (Farzi et al., 2015b (link)) and according to the manufacturer’s specifications.
Cytokines that were too low to be detected were excluded from further analysis. All cytokine concentrations were evaluated in duplicates. It should not go unmentioned at this point that IL-1β levels were analyzed in the blood plasma, but were too low to be detected throughout all groups and will not be displayed in the results part.
Corticosterone plasma levels were determined with a specific enzyme immunoassay kit (Assay Designs, Ann Arbor, MI, United States) with a sensitivity of 0.027 ng/ml as previously described (Farzi et al., 2015b (link)) and according to the manufacturer’s specifications.
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