The procedures were performed, as described elsewhere.57 (link),58 Briefly, before polymerization, fibrinogen solutions were mixed with fibrinogen conjugated with AlexaFluor-488 (Invitrogen, USA) at a ratio 50:1. Samples were prepared on slides and analyzed using a confocal laser scanning microscope LSM 880 equipped with an AiryScan module and GaAsP detector (Carl Zeiss, Germany; 40× water immersion objective).
Free full text: Click here