Longitudinal Analysis of T Cell Receptor Repertoires

All the rearranged T cell receptor β chain gene (TRB) repertoires analysed in this study have been generated by Adaptive Biotechnologies (Seattle, WA, USA) by high throughput sequencing following multiplex amplification of all possible V(D)J combinations. After sequencing, immunoSEQ Analyzer software (Adaptive Biotechnologies) was used to automatically annotate rearranged TRB genes and their complementary determining regions 3 (CDR3). A cross-sectional dataset of rearranged TRB gene repertoires generated from 587 donors aged from 1 to 74 years was downloaded in June 2017 from the Adaptive Biotechnologies website¹ (31 (link)). A second longitudinal dataset was obtained from 6 donors with informed consent, sampled 3 times each, around 10 years apart as described (25 (link)). The characteristics of both sets are provided in Table 1.

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Mika J., Yoshida K., Kusunoki Y., Candéias S.M, & Polanska J. (2023). Sex- and age-specific aspects of human peripheral T-cell dynamics. Frontiers in Immunology, 14, 1224304.

Publication 2023

immunoSEQ Analyzer software

Adaptive Donors Trb gene

Corresponding Organization :

Other organizations : Silesian University of Technology, Radiation Effects Research Foundation

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Variable analysis

independent variables

None explicitly mentioned.

dependent variables

Rearranged TRB gene repertoires generated from 587 donors aged from 1 to 74 years, and a longitudinal dataset from 6 donors sampled 3 times each, around 10 years apart.

control variables

None explicitly mentioned.

controls

No positive or negative controls were specified by the authors.

Annotations

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