Neutral comet assays were performed using previously described protocol with minor modifications35 (link). In brief, HCT116 cells were treated 20 μM SN38 for 2 h. Cells were then collected for neutral comet assays using the CometAssay Kit (R&D Systems, Catalog # 4250-050-K) following manufacturer’s instructions. Images were captured using BioSpa Live Cell Analysis System (Biotek) and tail moment was calculated using OpenComet70 (link), a plugin for the image processing program ImageJ.
Corresponding Organization : Center for Cancer Research
Other organizations :
National Center for Advancing Translational Sciences, National Institute of Biomedical Imaging and Bioengineering, The University of Texas Southwestern Medical Center
BioSpa Live Cell Analysis System (Biotek) for image capture
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
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