Immunofluorescence Assay for HHV-6 Antigens

Immunofluorescence for HHV-6 antigen expression was performed with a mouse monoclonal antibodies (mAb) directed against p41 and IE2 (early antigen) and glycoprotein gp116 (late antigen) of HHV-6 A and B (ABI, Columbia, MD, USA), as previously described [30 (link)]. Epithelial and stromal cells were respectively stained with mouse anti-Cytokeratin- 18, a heterotetramer cytoskeleton protein (CK18) and rabbit anti-vimentin, an intermediate filament of the cytoskeleton proteins moAbs (Abcam, Cambridge, UK), respectively. Nuclei were counterstained with DAPI (4,6-diamidino-2-phenylindole-dihydrochroride) (Sigma-Aldrich, S.Louis, MO, USA).

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Marci R., Gentili V., Bortolotti D., Lo Monte G., Caselli E., Bolzani S., Rotola A., Di Luca D, & Rizzo R. (2016). Presence of HHV-6A in Endometrial Epithelial Cells from Women with Primary Unexplained Infertility. PLoS ONE, 11(7), e0158304.

Publication 2016

mouse anti-Cytokeratin- 18 moAbs DAPI

Antigen Cytokeratin 18 Cytoskeleton Cytoskeleton protein Dapi Glycoprotein Hhv 6 Immunofluorescence Intermediate filament proteins Monoclonal antibodies Mouse Nuclei Rabbit Stromal cells Vimentin

Corresponding Organization : University of Ferrara

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Variable analysis

independent variables

Mouse monoclonal antibodies (mAb) directed against p41 and IE2 (early antigen) and glycoprotein gp116 (late antigen) of HHV-6 A and B

dependent variables

HHV-6 antigen expression

control variables

Epithelial cells stained with mouse anti-Cytokeratin-18 (CK18) antibody
Stromal cells stained with rabbit anti-vimentin antibody
Nuclei counterstained with DAPI (4,6-diamidino-2-phenylindole-dihydrochroride)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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