All cell lines were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Drug resistant cells SGC-R and BGC-R cells were developed from SGC7901 and BGC823, respectively 16 (link). All cell lines were cultured as previously reported. The chemical reagents including Dichloroacetic acid (DCA) (D54702), KNK437 (SML0964), Cycloheximide (R750107), chloroquine (C6628), MG132 (474790) and PD150606 (D5946) were purchased from Sigma Aldrich (USA). MLN4924 (B1036) and radicicol (A4067) were purchased from ApexBio (USA). Cisplatin and KRIBB11 (S840201) were obtained from Selleck (Shanghai, China).
Protocol detail
Drug Resistance Mechanism Research
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Cell lines
Cells
Chinese
Chloroquine
Cisplatin
Cycloheximide
Dichloroacetic acid
Drug
Knk437
Kribb11
Mg132
Mln4924
Pd150606
Radicicol
Corresponding Organization :
Other organizations : Zhejiang University, Sir Run Run Shaw Hospital, The First People's Hospital of Shunde, Southern Medical University, Chinese University of Hong Kong, Jena University Hospital
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Variable analysis
independent variables
- Dichloroacetic acid (DCA)
- KNK437
- Cycloheximide
- Chloroquine
- MG132
- PD150606
- MLN4924
- Radicicol
- Cisplatin
- KRIBB11
- Cell lines: SGC-R and BGC-R cells
- SGC7901 and BGC823 cell lines
- Positive control: SGC7901 and BGC823 cell lines
- Negative control: Not explicitly mentioned
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