Bacterial and Plasmid DNA Extraction

Genomic DNA extraction was carried out using the TaKaRa-MiniBEST Bacterial Genomic DNA Extraction Kit (Japan TaKaRa BIO, Dalian Company, Dalian, China). Plasmid DNA was extracted utilizing the TIANpure Midi Plasmid Kit (Tiangen Biotech Beijing Co. Ltd., Beijing, China). Construction of prophage gene deletion and complementary mutants for V. parahaemolyticus CHN25 followed a previous method outlined in our earlier studies [24 (link),25 (link)]. DNA sequencing was conducted by Sangon in China.

Free full text: Click here

Zhao H., Xu Y., Yang L., Wang Y., Li M, & Chen L. (2024). Biological Function of Prophage-Related Gene Cluster ΔVpaChn25_RS25055~ΔVpaChn25_0714 of Vibrio parahaemolyticus CHN25. International Journal of Molecular Sciences, 25(3), 1393.

Publication 2024

TIANpure Midi Plasmid Kit

Corresponding Organization : Shanghai Ocean University

Other organizations : Hunter Holmes McGuire VA Medical Center, Virginia Commonwealth University

Top 5 similar protocols

Variable analysis

independent variables

Genomic DNA extraction method (TaKaRa-MiniBEST Bacterial Genomic DNA Extraction Kit)
Plasmid DNA extraction method (TIANpure Midi Plasmid Kit)
Construction of prophage gene deletion and complementary mutants for V. parahaemolyticus CHN25

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!