Corticosterone Feeding Protocol

35 μg/ml corticosterone (Millipore Sigma, C2505) was dissolved in 0.45% hydroxypropyl-β-cyclodextrin and added to the drinking water during the entire corticosterone feeding period^{19 (link)}. corticosterone water was changed every 3 days to prevent degradation. Control animals received vehicle water (0.45% hydroxypropyl-β-cyclodextrin).

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Choi S., Zhang B., Ma S., Gonzalez-Celeiro M., Stein D., Jin X., Kim S.T., Kang Y.L., Besnard A., Rezza A., Grisanti L., Buenrostro J., Rendl M., Nahrendorf M., Sahay A, & Hsu Y.C. (2021). Corticosterone inhibits Gas6 to govern hair follicle stem cell quiescence. Nature, 592(7854), 428-432.

Publication 2021

corticosterone

Animals Corticosterone Cyclodextrin Hydroxypropyl

Corresponding Organization :

Other organizations : Harvard University, Harvard Stem Cell Institute, Icahn School of Medicine at Mount Sinai, Center for Systems Biology, Massachusetts General Hospital

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Variable analysis

independent variables

Corticosterone concentration: 35 μg/ml

dependent variables

Not explicitly mentioned

control variables

Hydroxypropyl-β-cyclodextrin concentration: 0.45%
Drinking water vehicle

controls

Positive control: Animals received corticosterone-containing drinking water
Negative control: Animals received vehicle-only drinking water (0.45% hydroxypropyl-β-cyclodextrin)

Annotations

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