The purified samples (5 g) of each were taken into different flasks. For oxidation, 375 mL of 0.03 M periodic acid (sodium metaperiodate and sulfuric acid) was added in 5 g of each dried cellulosic material. Then the pH of the solution was adjusted to 3.0 using sulfuric acid. These solutions were kept in a shaking incubator at 60°C and 150 rpm for 24 h. After 24 h these materials were filtered and washed with distilled water several times [20 ]. After completion of washing, the three materials were transferred in separate petri plates, and then dried in a hot air oven for 48 to 72 h.
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