Pulmonary Vascular Remodeling Evaluation

Pulmonary vascular remodeling was evaluated as previously described [36 (link)]. Briefly, lung sections were stained with polyclonal rabbit anti-human vWF (Dako), mouse anti α-smooth muscle actin (SMA,1:500; Sigma, St Louis, MO) and 4′,6-diamidino-2-phenylindole (DAPI, Vector Biolabs, Burlingame, CA). The percentage of peripheral pulmonary vessels (< 50 μm in diameter) stained with α-SMA > 50% of the circumference was determined from ten random images on each lung section and all analyses were performed by a blinded observer.

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Guerra K., Bryan C., Dapaah-Siakwan F., Sammour I., Drummond S., Zambrano R., Chen P., Huang J., Sharma M., Shrager S., Benny M., Wu S, & Young K.C. (2019). Intra-tracheal administration of a naked plasmid expressing stromal derived factor-1 improves lung structure in rodents with experimental bronchopulmonary dysplasia. Respiratory Research, 20, 255.

Publication 2019

polyclonal rabbit anti-human vWF mouse anti α-smooth muscle actin (SMA,

Dapi Human Lung Mouse α smooth muscle actin Rabbit Sma 1 Vector Vessels

Corresponding Organization :

Other organizations : University of Miami

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Variable analysis

independent variables

Lung sections were stained with polyclonal rabbit anti-human vWF (Dako), mouse anti α-smooth muscle actin (SMA,1:500; Sigma, St Louis, MO) and 4′,6-diamidino-2-phenylindole (DAPI, Vector Biolabs, Burlingame, CA)

dependent variables

The percentage of peripheral pulmonary vessels (< 50 μm in diameter) stained with α-SMA > 50% of the circumference

control variables

Ten random images on each lung section
All analyses were performed by a blinded observer

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