CD spectra were recorded on CD spectropolarimeter (Jasco J‐1500), equipped with a Peltier‐type temperature controller, as previously described (Dindo et al., 2020 (link); Pedretti et al., 2020 (link)). Briefly, near UV–Vis (250–600 nm) spectra of 1 mg/mL TgCGL were collected in 1‐cm path length quartz cuvette at a scan speed of 50 nm/min in 20 mM sodium phosphate pH 8 at 25°C. A minimum of three accumulations were made for each scan, averaged, and corrected for the blank solution of corresponding buffer (Bombardi et al., 2020 (link)).