Synthesis of Carboxylic Acid-Functionalized Gold Nanoparticles

Following a previously reported protocol, the 40 nm gold nanoparticles (GNPs) were synthesized using trisodium citrate as a reducing agent [27 (link)]. Briefly, a mixture of 1 mM gold(III) chloride trihydrate and 0.1 nM mPEG-carboxymethyl was heated with constant stirring, and 2.5 mL of 34 mM trisodium citrate was added while boiling. After 1 min of continuous boiling, the mixture was cooled to room temperature to obtain carboxylic acid-functionalized 40 nm GNPs, which were used to prepare mAb-GNP probes.

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Wu S.W., Chen Y.J., Chang Y.W., Huang C.Y., Liu B.H, & Yu F.Y. (2024). Novel enzyme-linked aptamer-antibody sandwich assay and hybrid lateral flow strip for SARS-CoV-2 detection. Journal of Nanobiotechnology, 22, 5.

Publication 2024

Corresponding Organization :

Other organizations : National Taiwan University, Chung Shan Medical University, Chung Shan Medical University Hospital

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Variable analysis

independent variables

Concentration of gold(III) chloride trihydrate (1 mM)
Concentration of mPEG-carboxymethyl (0.1 nM)
Concentration of trisodium citrate (34 mM)

dependent variables

Size of synthesized gold nanoparticles (GNPs) (40 nm)

control variables

Heating with constant stirring
Duration of boiling (1 min)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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