Extraction of total protein extracts and analysis by Western immunoblotting and coIP was recently described in detail [66 (link)]. Proteins for coIP experiments were isolated 48 h after siRNA transfection. Antibodies used in this study are listed in Suppl. Table S2. Signal detection for coIP experiments was done using the Quick Western Kit (LI-COR Biosciences, Bad Homburg, Germany).
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