MdMYB10 Transgenic Apple Generation

The binary vector pSAK277-MdMYB10 (Figure S5) containing the MdMYB10 cDNA driven by the 35S promoter was transferred into Agrobacterium tumefaciens strain GV3101 by the freeze–thaw method. Transgenic Malus domestica‘Royal Gala’ plants were generated by Agrobacterium-mediated transformation of leaf pieces, using a method previously reported (Yao et al., 1995 ).

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Espley R.V., Hellens R.P., Putterill J., Stevenson D.E., Kutty-Amma S, & Allan A.C. (2007). Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. The Plant Journal, 49(3), 414-427.

Publication 2007

Agrobacterium Agrobacterium tumefaciens Cdna Freeze method Gala Leaf Malus domestica Strain Transgenic plants Vector

Corresponding Organization : AgResearch

Other organizations : University of Auckland

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Variable analysis

independent variables

Transfer of the binary vector pSAK277-MdMYB10 containing the MdMYB10 cDNA driven by the 35S promoter into Agrobacterium tumefaciens strain GV3101

dependent variables

Generation of transgenic Malus domestica 'Royal Gala' plants by Agrobacterium-mediated transformation of leaf pieces

control variables

Transformation method (previously reported by Yao et al., 1995)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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