Chromatin immunoprecipitation (ChIP) assays using IgG (Upstate, Boston, USA) or antibodies specific to NFATc2, H3K4me3 (all Abcam, Cambridge, UK), RNA Polymerase II (Millipore, Billerica, USA) or STAT3 (Cell Signaling, Danvers, USA) were performed as previously described (27 (link)). Bound regions were detected by q-PCR using the following primer: CDK 6 promoter: 5´-tcccgttccactgcgtcc-3´, 5´-caagccgcttaatccttcctggtt-3´ (Biomers).
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ChIP-qPCR Analysis of Transcription Factors
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Corresponding Organization :
Other organizations : Philipps University of Marburg, Mayo Clinic, University of Illinois at Chicago, St. Joseph's Hospital and Medical Center
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Variable analysis
independent variables
- Antibodies specific to NFATc2, H3K4me3, RNA Polymerase II, or STAT3
- Bound regions detected by q-PCR using primers for the CDK 6 promoter
- IgG (Upstate, Boston, USA)
- Positive control: IgG (Upstate, Boston, USA)
- Negative control: Not explicitly mentioned
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