Intravenous CIRP administration in hemorrhaged rats

One ml of rmCIRP or normal saline (vehicle) was administered intravenously to healthy animals. Antisera to CIRP, rabbit control IgG, or vehicles were administered to hemorrhaged rats 15 min after the initiation of fluid resuscitation over a period of 45 min via the femoral venous catheter.

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Qiang X., Yang W.L., Wu R., Zhou M., Jacob A., Dong W., Kuncewitch M., Ji Y., Yang H., Wang H., Fujita J., Nicastro J., Coppa G.F., Tracey K.J, & Wang P. (2013). Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis. Nature medicine, 19(11), 1489-1495.

Publication 2013

Animals Antisera Catheter Femoral venous Normal saline Rabbit Rats Resuscitation

Corresponding Organization :

Other organizations : Feinstein Institute for Medical Research, Kyoto University

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Variable analysis

independent variables

RmCIRP
Normal saline (vehicle)

dependent variables

Survival of hemorrhaged rats

control variables

Healthy animals
Hemorrhaged rats

controls

Positive control: Antisera to CIRP
Negative control: Rabbit control IgG
Vehicle control: Vehicles administered to hemorrhaged rats

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