Ca2+ transients reported by GCaMP6s were imaged over 6 weeks post AAV injection (ISOI/2p calibration experiments) or at two developmental time points, P20 and P36, in GCaMP6s expressing transgenic mice, using a custom-built two-photon microscope using galvanometer-based scan mirrors (6mm diameter, Cambridge Technologies) with a 16 x magnification and 0.8 numerical aperture water immersion objective (Nikon) and a large aperture collection pathway with GaAsP photomultiplier tubes (Hamamatsu). Frame scans (1 frame/s or 15.6 frames/s) were acquired using ScanImage51 (link).
Protocol detail
Longitudinal Calcium Imaging in Mice
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Corresponding Organization : University of North Carolina at Chapel Hill
Other organizations : Imaging Center
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Variable analysis
independent variables
- Time post AAV injection (6 weeks)
- Developmental time points (P20 and P36)
- Ca2+ transients reported by GCaMP6s
- Custom-built two-photon microscope using galvanometer-based scan mirrors (6mm diameter, Cambridge Technologies)
- 16x magnification and 0.8 numerical aperture water immersion objective (Nikon)
- Large aperture collection pathway with GaAsP photomultiplier tubes (Hamamatsu)
- Frame scans (1 frame/s or 15.6 frames/s) acquired using ScanImage51
- No positive or negative controls explicitly mentioned
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