Single cell suspensions were prepared from dorsal skin as previously described (12 (link)), and skin tissue was sliced into smaller pieces and digested for 1.5 hours at 37 °C with a solution of RPMI + 10mM Hepes + 0.05% DNase I (Sigma-Aldrich) + 0.27% collagenase type XI (Sigma-Aldrich) + 0.027% hyaluronidase type IV-S (Sigma-Aldrich).
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Isolation of Single Skin Cells
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Corresponding Organization : University of Alabama at Birmingham
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Variable analysis
independent variables
- Tissue slicing into smaller pieces
- Digestion time (1.5 hours)
- Digestion temperature (37 °C)
- Digestion solution composition (RPMI + 10mM Hepes + 0.05% DNase I + 0.27% collagenase type XI + 0.027% hyaluronidase type IV-S)
- Not explicitly mentioned
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the provided information.
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