CTC-matched formalin-fixed, paraffin-embedded tumor tissues were
evaluated by RNA in situ hybridization for CTNNB1 expression
(Hs-CNNTB1 probe #311731) using the RNAscope Multiplex Fluorescent Kit V2
(Advanced Cell Diagnostics) according to the manufacturer’s instructions.
Immunofluorescence staining was observed under the fluorescence microscope
(Nikon) and representative images were taken for each condition. Assays for
formalin-fixed, paraffin-embedded tissues were developed and optimized as
previously described (19 (link)). Images were
analyzed based on ACD guidelines for in situ hybridization scoring. 3-plex
negative (#320871) and positive (#320861) probes served as controls and target
bacterial dihydrodipicolinate reductase gene and housekeeping genes,
respectively. In situ hybridization staining images for specific genes were
assessed only when proper positive and negative controls were identified.
evaluated by RNA in situ hybridization for CTNNB1 expression
(Hs-CNNTB1 probe #311731) using the RNAscope Multiplex Fluorescent Kit V2
(Advanced Cell Diagnostics) according to the manufacturer’s instructions.
Immunofluorescence staining was observed under the fluorescence microscope
(Nikon) and representative images were taken for each condition. Assays for
formalin-fixed, paraffin-embedded tissues were developed and optimized as
previously described (19 (link)). Images were
analyzed based on ACD guidelines for in situ hybridization scoring. 3-plex
negative (#320871) and positive (#320861) probes served as controls and target
bacterial dihydrodipicolinate reductase gene and housekeeping genes,
respectively. In situ hybridization staining images for specific genes were
assessed only when proper positive and negative controls were identified.
