Detecting ESBL/AmpC E. coli from Livestock

Extended-spectrum β-lactamases/AmpC-producing E. coli strains derived from the strain collection of the Institute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin. The isolates were obtained from 7 different pig farms (n = 104) and 22 turkey farms (n = 51) in Germany. Each isolate originated from an individual sample including feces, boot swabs, manure, air or dust. ESBL/AmpC E. coli were selected using MacConkey agar (Oxoid, CM 0115, Wesel, Germany) supplemented with 1 mg/L cefotaxime, followed by species confirmation using MALDI-TOF identification (MALDI Microflex LT and Biotyper database, Bruker Daltonics, Bremen, Germany). The presence of the β-lactamase genes bla_CTX, bla_TEM, bla_SHV and the CIT-type AmpCs (e.g., CMY-2) was confirmed by real-time PCR as described by Roschanski et al. (2014) (link).

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Skaradzińska A., Śliwka P., Kuźmińska-Bajor M., Skaradziński G., Rząsa A., Friese A., Roschanski N., Murugaiyan J, & Roesler U.H. (2017). The Efficacy of Isolated Bacteriophages from Pig Farms against ESBL/AmpC-Producing Escherichia coli from Pig and Turkey Farms. Frontiers in Microbiology, 8, 530.

Publication 2017

CM 0115 MALDI Microflex LT Biotyper database

Agar Animal Cefotaxime E coli Feces Genes Maldi Real time pcr Strain β lactamase

Corresponding Organization :

Other organizations : Wroclaw University of Environmental and Life Sciences, Freie Universität Berlin

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Variable analysis

independent variables

Pig farms (n = 104)
Turkey farms (n = 51)

dependent variables

Presence of extended-spectrum β-lactamases (ESBL) or AmpC-producing E. coli strains

control variables

MacConkey agar (Oxoid, CM 0115, Wesel, Germany) supplemented with 1 mg/L cefotaxime for ESBL/AmpC E. coli selection
MALDI-TOF identification (MALDI Microflex LT and Biotyper database, Bruker Daltonics, Bremen, Germany) for species confirmation
Real-time PCR to confirm the presence of β-lactamase genes (bla_CTX, bla_TEM, bla_SHV, and CIT-type AmpCs such as CMY-2)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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