Immunofluorescent Exosome Tracking in Cells

For immunofluorescence Staining, the mainly procedure was described in previously report (Zhang et al., 2022 (link)). Briefly, the treated Hela/DDP and Caski/DDP cells were washed by PBS and fixed by ice chilled methanol. The exosome was labeled by PKH26 (Puzar Dominkus et al., 2018 (link)) using PKH26 Red Fluorescent Cell Linker Kits for General Cell Membrane Labeling (Sigma-Aldrich). DAPI (Sigma-Aldrich) stained cellular nucleus with following the instruction, slides were imaged by confocal laser scanning microscopy (Olympus).

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Zhou J., Wang Y., Zhang L., Chen Q., Zhu X., Jiang P., Jiang N., Zhao W, & Li B. (2022). Engineered Exosomes-Mediated Transfer of hsa-miR-320a Overcomes Chemoresistance in Cervical Cancer Cells via Targeting MCL1. Frontiers in Pharmacology, 13, 883445.

Publication 2022

PKH26 Red Fluorescent Cell Linker Kits for General Cell Membrane Labeling DAPI confocal laser scanning microscopy

Cells Cellular nucleus Confocal laser scanning microscopy Dapi Exosome Hela Immunofluorescence Methanol Pkh26 Red cell membrane

Corresponding Organization : City University of Hong Kong

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Variable analysis

independent variables

Treated Hela/DDP and Caski/DDP cells

dependent variables

Immunofluorescence staining of exosomes

control variables

PBS washing
Methanol fixation
DAPI staining of cellular nucleus

controls

No positive or negative controls were explicitly mentioned.

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