The method previously described by Sinha (22) was applied to determine CAT activity with reagents including 30 mM H2O2, phosphate buffer (50 mM; pH 7.4), dichromate/acetic acid solution (5% aqueous potassium dichromate solution in distilled water, and 150 mL of Glacial (98-100%) acetic acid). In this technique, the reduction of dichromate in acetic acid to chromic acetate occurs on heating with H 2 O 2 , and the resulting chromic acetate was measured at 570 nm using a spectrophotometer.
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Catalase Activity Determination via Dichromate
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Other organizations : Kerman University of Medical Sciences
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Variable analysis
independent variables
- H2O2 concentration (30 mM)
- CAT activity
- Phosphate buffer (50 mM; pH 7.4)
- Dichromate/acetic acid solution (5% aqueous potassium dichromate solution in distilled water, and 150 mL of Glacial (98-100%) acetic acid)
- No positive or negative controls were explicitly mentioned.
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