Drosophila Genetic Interaction Analysis

Drosophila were raised on standard cornmeal–yeast–sucrose medium at 25°C on a 12 h light:dark cycle. CHMP2B^Intron5 flies were described previously (7 (link),10 (link)). All other stocks were obtained from the following sources: POSH⁷⁴ (Toshiro Aigaki, Tokyo Metropolitan University, Japan) (29 (link)), AKT¹ (Clive Wilson, University of Oxford, UK) (25 (link)), OK6-Gal4 (Cahir O’Kane, University of Cambridge, UK), UAS-myrAKT, UAS-mCD8-GFP, AKT⁰⁴²²⁶, AKT³, UAS-AKT-RNAi (BL #33615), UAS-mCherry-POSH, UAS-POSH-RNAi (BL #64569), Df(3L)H99, Puc-LacZ, glass multimer reporter (GMR)-Gal4, nSyb-Gal4, Canton S, w¹¹¹⁸ (Bloomington Stock Center). UAS-AKT (FlyORF, Zurich, Switzerland). All wild-types were an outcross of Canton S to w¹¹¹⁸.
Genetic interaction experiments and quantification of the CHMP2B^Intron5 eye phenotype was performed as described previously (7 (link)). Eyes were imaged using an AxioCam ERc 5s camera (Carl Zeiss) mounted on a Stemi 2000-C stereo microscope (Carl Zeiss).