Prostate Cancer Cell Lines for Binding Studies

The human prostate cancer cell lines C4-2 (PSMA-positive, ATCC^® CRL-3314™) [69 (link)] and PC-3 (PSMA-negative, ATCC^® CRL-1435™) [70 (link)] were obtained from ATCC (Manassas, Virginia). Cells were grown in RPMI 1640 medium (Merck Norge, Oslo, Norway) supplemented with 10% heat-inactivated foetal bovine serum (GE Healthcare Life Sciences, Chicago, IL, USA) and 100 units/mL penicillin and 100 µg/mL streptomycin (Sigma-Aldrich) at 37 °C in a humid atmosphere of 95% air and 5% CO₂. Before injection of radiolabelled ligands in mice, binding of the radioligands was verified by measuring cell binding ability in C4-2 cells, as described previously [25 (link)].