The glycogen levels in soleus muscle samples were measured using a glycogen colorimetric assay kit (BioVision) according to the manufacturer’s instructions. Soleus was selected as the target muscle for this study as studies have shown that soleus muscle is one the key muscle types that can be improved with swimming training in rat model (Forte et al., 2020 (link); Moustafa and Arisha, 2020 (link)).
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