Total RNA was extracted using a MiniBEST Plant RNA Extraction Kit (Takara Biotechnology Inc., Kusatsu, Japan). Approximately 100 mg of fresh mycelia was ground in liquid nitrogen before extraction. The RNA quality and quantity were determined using the Thermo Scientific NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
RNA-seq of the S. bambusicola (GDMCC 60438) mycelia at three different fermentation temperatures was performed by Annoroad Gene Technology Co., Ltd. (Beijing, China), and three replicates for each sample were used for RNA extraction. A cDNA library was constructed for each replicate, and the libraries were sequenced on an MGI T7 platform (BGI Inc., Shenzhen, China) followed by paired-end 150-bp read generation. Then, the low-quality reads were filtered out using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ) and Skewer (https://sourceforge.net/projects/skewer ) with the parameters set to -q 20 -Q 30 -l 50 [27 (link)].
RNA-seq of the S. bambusicola (GDMCC 60438) mycelia at three different fermentation temperatures was performed by Annoroad Gene Technology Co., Ltd. (Beijing, China), and three replicates for each sample were used for RNA extraction. A cDNA library was constructed for each replicate, and the libraries were sequenced on an MGI T7 platform (BGI Inc., Shenzhen, China) followed by paired-end 150-bp read generation. Then, the low-quality reads were filtered out using FastQC (
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