The study of IgG and IgM aCL and aβ2GPI was performed by chemiluminescence assay (CIA) (QUANTA Flash®, Inova Diagnostics, CA). The cut-off recommended by the manufacturer was 20 chemiluminescent units (CU). However, the low/medium cut-off point (the equivalent of < 40 GPL and MPL units) was defined as 95 CU for IgG aCL and 31 for IgM aCL as previously established (12 (link)).
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