Bracts and apical meristem sections were prepared as described above. ROS detection was performed by staining with DAB [67 (link),68 (link)] (Sigma-Aldrich, Shanghai, China) and HPF [69 (link),70 (link)] (Maokang Bio-technology, Shanghai, China). The samples were examined and captured immediately using a ZEISS Axio Imager 2 microscope (Carl Zeiss, Germany).
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