Ferroverdin A Quantification by HPLC

Ferroverdin extraction with ethyl acetate, drying, and resuspension in acetonitrile were performed as described previously [19 (link)]. Samples were analyzed by HPLC and Ferroverdin A was detected at 440 nm and quantified by peak area integration, as described previously [19 (link)]. Data were analyzed using Empower 3 (Waters, Milford, MA, USA). The HPLC-based protocol for ferroverdin A semi-quantitative analysis is detailed in Appendix A.

Free full text: Click here

Martinet L., Naômé A., Baiwir D., De Pauw E., Mazzucchelli G, & Rigali S. (2020). On the Risks of Phylogeny-Based Strain Prioritization for Drug Discovery: Streptomyces lunaelactis as a Case Study. Biomolecules, 10(7), 1027.

Publication 2020

Empower 3

Acetonitrile Ethyl acetate Ferroverdin Hplc

Corresponding Organization : University of Liège

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Ferroverdin extraction with ethyl acetate

dependent variables

Ferroverdin A detected at 440 nm
Ferroverdin A quantified by peak area integration

control variables

Drying
Resuspension in acetonitrile

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!