The
desolvation technique was used to fabricate gum nanoparticles. The
gum nanoparticles were prepared through using a desolvation method
by dropwise addition of the desolvating agent (ethanol) continuously.
The modified version of the method described by Taheri et al.23 (link) was used in this study.22 (link) The gum solutions (solvent) were blended at 800 rpm for 5 min. OPE
(0.1%) and Tween 20 (0.5%) were added in optimized amounts of ethanol
(antisolvent). Tween 20 was used for the better dissolving of OPE
in ethanol. Ethanolic OPE (0.5 mL/min) was put dropwise to the gum
solution (solvent phase) using a syringe pump system (New Era, NE,
USA). After adding the ethanol, the solution was stirred at 800 rpm
for 10 min. Then, ultrasonication (Hielscher UIP1000hdT, Germany)
of 100 W was performed to the solutions for 1 min (every 30 s wait
for 10 s) in an ice bath. The nanoparticle suspensions were centrifuged
at 9000 rpm for 30 min. Nanoparticles were redispersed with 5 mL of
distilled water and then freeze-dried without using cryoprotectants.
The same experimental analysis without OPE and Tween 20 was performed
for blank gum nanoparticle fabrication.
desolvation technique was used to fabricate gum nanoparticles. The
gum nanoparticles were prepared through using a desolvation method
by dropwise addition of the desolvating agent (ethanol) continuously.
The modified version of the method described by Taheri et al.23 (link) was used in this study.22 (link) The gum solutions (solvent) were blended at 800 rpm for 5 min. OPE
(0.1%) and Tween 20 (0.5%) were added in optimized amounts of ethanol
(antisolvent). Tween 20 was used for the better dissolving of OPE
in ethanol. Ethanolic OPE (0.5 mL/min) was put dropwise to the gum
solution (solvent phase) using a syringe pump system (New Era, NE,
USA). After adding the ethanol, the solution was stirred at 800 rpm
for 10 min. Then, ultrasonication (Hielscher UIP1000hdT, Germany)
of 100 W was performed to the solutions for 1 min (every 30 s wait
for 10 s) in an ice bath. The nanoparticle suspensions were centrifuged
at 9000 rpm for 30 min. Nanoparticles were redispersed with 5 mL of
distilled water and then freeze-dried without using cryoprotectants.
The same experimental analysis without OPE and Tween 20 was performed
for blank gum nanoparticle fabrication.
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