Ambion TRIZOL reagent Kit (by Life Technologies Invitrogen, CA, USA): Total RNA was extracted according to the manufacturer's instructions. Extraction buffer used in this method contains guanidium thiocyanate and RNA was precipitated in isopropanol.
RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA): RNA was extracted according to the manufacturer's instructions. The method uses guanidium thiocyanate based lysis buffer and RNA purification was done using silica-membrane column.
Furtado6 method: The total RNA was isolated based on the combination of both TRIZOL and RNeasy Plant Mini Kit (Qiagen) method. RNA extraction was carried out in the TRIZOL reagent, and the collected aqueous phase was extracted in RLC buffer, followed by steps in RNeasy Plant Mini Kit manufactures method.
CTAB-LiCl method: RNA was extracted based on the method described by White et al.3 (link). In this method, ionic detergent cetyltrimethylammonium bromide (CTAB) was used in the extraction buffer, and LiCl was used to precipitate RNA.
New Modified method (SDS-LiCl): This method follows the SDS-Phenol based extraction and RNA precipitation with sodium acetate and LiCl (Fig.1 ).![]()
RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA): RNA was extracted according to the manufacturer's instructions. The method uses guanidium thiocyanate based lysis buffer and RNA purification was done using silica-membrane column.
Furtado6 method: The total RNA was isolated based on the combination of both TRIZOL and RNeasy Plant Mini Kit (Qiagen) method. RNA extraction was carried out in the TRIZOL reagent, and the collected aqueous phase was extracted in RLC buffer, followed by steps in RNeasy Plant Mini Kit manufactures method.
CTAB-LiCl method: RNA was extracted based on the method described by White et al.3 (link). In this method, ionic detergent cetyltrimethylammonium bromide (CTAB) was used in the extraction buffer, and LiCl was used to precipitate RNA.
New Modified method (SDS-LiCl): This method follows the SDS-Phenol based extraction and RNA precipitation with sodium acetate and LiCl (Fig.
Flow chart describing the major steps involved in the total RNA extraction using the modified SDS-LiCl method. Information for each step (numbers in parenthesis) is detailed in the procedure section.
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