Maintenance of Pluripotent and Differentiated hESCs

MAVS^+/+ hESCs (derived from Line H9, WiCell Research Institute) and MAVS^−/− hESCs grown on mouse embryonic fibroblast (MEF) feeder layers inactivated with mitomycin C were cultured with conventional DMEM/F12 (Gibco) culture medium [42 (link)]. hESCs were also maintained on precoated plates with Matrigel (BD Biosciences) using mTeSR medium. hESC-derived hMSCs were maintained with hMSC culture medium [43 (link)]. hNSCs were cultured according to a previous study [71 (link)].

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Wang C., Yang K., Liu X., Wang S., Song M., Belmonte J.C., Qu J., Liu G.H, & Zhang W. (2023). MAVS Antagonizes Human Stem Cell Senescence as a Mitochondrial Stabilizer. Research, 6, 0192.

Publication 2023

Line H9 DMEM/F12 Matrigel

Culture medium Embryonic Feeder layers Fibroblast Hesc Matrigel Mavs Mitomycin c Mouse

Corresponding Organization :

Other organizations : Beijing Institute of Genomics, University of Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Capital Medical University, Center for Excellence in Molecular Cell Science

Top 5 similar protocols

Variable analysis

independent variables

MAVS^+/+
MAVS^−/−

dependent variables

Growth of hESCs
Growth of hMSCs
Growth of hNSCs

control variables

Mouse embryonic fibroblast (MEF) feeder layers inactivated with mitomycin C
Matrigel (BD Biosciences) precoated plates
MTeSR medium
HMSC culture medium

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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