Western Blotting and Immunohistochemical Assays

The western blotting analysis was performed following the methods of a previous study (37 (link)). Equal amounts (30 μg) of protein were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 10% gel. The following primary antibodies used for the western blotting analysis were purchased from Proteintech Group (Rosemont, United States): anti-DNAJB1 (Catalog number: 13174-1-AP; 1:1000): and anti-alpha tubulin (Catalog number: 11224-1-AP; 1:3000) antibodies. The immunohistochemical assay was performed following the methods of a previous study (38 (link)).