The RNA isolation was performed in peripheral blood samples of all study groups using the AccuZol™ Total RNA extraction kit (Bioneer Corp., Daejeon, South Korea), and the RNAs were translated to complementary deoxyribonucleic acid (cDNA) with high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific, MA, USA).
Each cDNA sample was analyzed for gene expressions in ExiCycler™ 96 Quantitative-PCR device (Bioneer Corp., Daejeon, South Korea) by RT-PCR using the K-6253 AccuPower® 2X GreenStar™ Master Mix kit (Bioneer Corp., Daejeon, South Korea) and specific primers designed with reference to the literature on DICER1 (Dicer), Drosha, DGCR8, XPO5, and AGO2 (Table 1 ).[16 (link)-18 (link)]
Each cDNA sample was analyzed for gene expressions in ExiCycler™ 96 Quantitative-PCR device (Bioneer Corp., Daejeon, South Korea) by RT-PCR using the K-6253 AccuPower® 2X GreenStar™ Master Mix kit (Bioneer Corp., Daejeon, South Korea) and specific primers designed with reference to the literature on DICER1 (Dicer), Drosha, DGCR8, XPO5, and AGO2 (
