Protocol detail

Quantifying Immunohistochemistry in Glioma Tissues

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Glioma tissues were formalin-fixed, paraffin-embedded, and sectioned at a thickness of 4 μm. Immune complexes were detected with the SP Kit (Solarbio, Beijing, China) and DAB Substrate Kit (Solarbio, Beijing, China). Signals were detected using Olympus BX41 microscope. Immunohistochemistry was performed using standard protocols as described previously (24 (link)). Quantification of Immunohistochemistry (IHC) staining was performed in a blinded fashion. ImageJ and ImageJ plugin IHC profiler was applied to quantify IHC staining analysis as reported (25 (link)).

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Dong J., Wang F., Xu Y., Gao X., Zhao H., Zhang J., Wang N., Liu Z., Yan X., Jin J., Ji H., Cheng R., Wang L., Qiu Z, & Hu S. (2023). Using mixed reality technique combines multimodal imaging signatures to adjuvant glioma photodynamic therapy. Frontiers in Medicine, 10, 1171819.

Publication 2023

SP Kit DAB Substrate Kit BX41 microscope

Formalin Glioma Immune complexes Microscope Paraffin Tissues

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Immunohistochemistry (IHC) staining signals

control variables

Tissue preparation (formalin-fixed, paraffin-embedded, and sectioned at 4 μm thickness)
Detection of immune complexes using the SP Kit and DAB Substrate Kit
Signal detection using Olympus BX41 microscope
Immunohistochemistry protocol as described previously (24)

controls

No positive or negative controls were explicitly mentioned in the input.

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