Acetamiprid Exposure in Cockroaches

Acetamiprid was applied to cockroaches in three different ways at concentrations of 0.1, 0.5, and 1 nmol.g⁻¹ [22 (link)]. For topical application, 1 µL of the final solution was applied to the thorax (dorsal surface) of the cockroach using a Hamilton syringe. As previously described, for the haemolymph treatment, 10 µL of solution was injected between the third and fourth sternites [25 (link)]. Oral application consisted of a starvation for the day before the experiment, and then each cockroach was fed with 5 µL solution (either acetamiprid or control solution) [22 (link)]. For each condition, acetamiprid was administered to the cockroaches one time the day before the experiment.