Characterization of Mesenchymal Stem Cells

Cell preparation, antibody staining and flow cytometry were performed as described previously [8 (link)–10 (link), 31 (link)]. Briefly, the MSCs were detached with TrypLE, resuspended in PBS plus 5% BSA (bovine serum albumin, Sigma) and incubated with anti-CD317-PE (Thermo Fisher Scientific), anti-CD73-FITC, anti-CD90-FITC, anti-CD105-FITC, anti-CD45-FITC, anti-CD34-FITC, anti-CD19-FITC, anti-CD11b-FITC, anti-HLADR-FITC, IgG-PE or IgG-FITC (all from BD Biosciences). Data were collected with BD AccuriC6 Plus (BD Biosciences) and analyzed with FlowJo software.

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Song J., Ma Q., Li Y., Wang X., Chen S., Liang B., Lin X., Chen J., Xu S., Shi S., Zhang J., Diao L., Zeng Y, & Xu J. (2024). CD317+ MSCs expanded with chemically defined media have enhanced immunological anti-inflammatory activities. Stem Cell Research & Therapy, 15, 2.

Publication 2024

BSA anti-CD317-PE anti-CD90-FITC anti-CD105-FITC anti-CD45-FITC anti-CD34-FITC anti-CD19-FITC anti-CD11b-FITC anti-HLADR-FITC BD AccuriC6 Plus

Corresponding Organization :

Other organizations : Northeast Agricultural University, Shenzhen Zhongshan Urological Hospital, Shenzhen University, Shenzhen Bao'an District People's Hospital

Top 5 similar protocols

Variable analysis

independent variables

Cell preparation, antibody staining and flow cytometry

dependent variables

CD317-PE expression
CD73-FITC expression
CD90-FITC expression
CD105-FITC expression
CD45-FITC expression
CD34-FITC expression
CD19-FITC expression
CD11b-FITC expression
HLADR-FITC expression

control variables

PBS plus 5% BSA
IgG-PE
IgG-FITC

positive controls

IgG-PE
IgG-FITC

negative controls

IgG-PE
IgG-FITC

Annotations

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