Fetal Tissue Histological Preparation

Fetuses were fixed over several days in Bouin's solution (Sigma-Aldrich) or in 4% paraformaldehyde in PBS, dehydrated in an ethanol series and embedded in paraffin wax. Sections (5 µm thickness) were stained with Harris' haematoxylin solution and 2% Eosin Y, or Alizarin Red and Fast Green (all Sigma-Aldrich). Images were captured on an Axiophot2 upright microscope. For skull preparations, fetal heads were skinned and stained with Alizarin Red (0.15%) in 1% KOH and cleared with 1% KOH in 20% glycerol (Peskett et al., 2017 (link)).

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Rolo A., Galea G.L., Savery D., Greene N.D, & Copp A.J. (2019). Novel mouse model of encephalocele: post-neurulation origin and relationship to open neural tube defects. Disease Models & Mechanisms, 12(11), dmm040683.

Publication 2019

Bouin's solution Harris' haematoxylin solution Eosin Y

Bouin solution Dehydrated ethanol Eosin y Fast green Fetal Fetuses Glycerol Heads Microscope Paraffin wax Paraformaldehyde Skull

Corresponding Organization :

Other organizations : University College London

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Variable analysis

independent variables

Fixation method: Bouin's solution (Sigma-Aldrich) or 4% paraformaldehyde in PBS

dependent variables

Histological staining: Harris' haematoxylin solution and 2% Eosin Y, or Alizarin Red and Fast Green
Imaging: Images captured on an Axiophot2 upright microscope

control variables

Tissue preparation: Dehydration in an ethanol series and embedding in paraffin wax
Skull preparation: Fetal heads were skinned and stained with Alizarin Red (0.15%) in 1% KOH and cleared with 1% KOH in 20% glycerol

positive controls

None specified

negative controls

None specified

Annotations

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