Protein Expression Analysis Protocol

The protein concentration was determined strictly in accordance with the Pierce BCA Assay Kit (Thermo Scientific, USA) instructions. Samples were electrophoresed on 12% Bis–Tris Gel/MOPS (Invitrogen, NY, USA) for 2 h, and transferred to PVDF membranes (Immobilon, Millipore, USA) for 1.5 h. The membranes were incubated with 5% skimmed milk (2321000, BD, USA) for 1.5 h, incubated with the primary and secondary antibodies (Table S2). The antibodies for GAPDH^{9 (link)}, Bcl-2^{9 (link)}, Bax^{9 (link)}, β-catenin^{9 (link)}, and p7TP3^{17 (link)} has been used in previous experiments. The interested bands were analyzed by BiolD software (Vilber Lourmat, France). The blots were cut prior to hybridisation with antibodies. Therefore, original images of full-length blots cannot be provided. We have provided all original blots, with membrane edges visible, in supplementary materials.