Masson’s trichrome stain kit (G1340, Beijing Solarbio Science and Technology, Beijing, China) was used to stain the prepared paraffin sections (G1340, Beijing Solarbio Science and Technology, Beijing, China). The Masson’s trichrome stain slices were observed by the optical microscope (Leica CTR6, Leica, Germany). ImageJ software (version 1.8.0) was used to quantify the collagen proportion of stained pieces.
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