Western blotting was performed using the Multistrip Western blotting protocol as previously described [16 (link)]. The following antibodies were utilized: The exosomes were identified by CD63 (1:1000; Abcam), CD9 (1:1000; Abcam), TSG101 (1:1000; Abcam). The expression of Nrf2/GPX4 pathway was detected by Nrf2 (1:1000;Cell Signaling Technology), xCT (1:1000; Abcam), GPX4 (1:1000; Abcam), and Alpha Tubulin (1:200; Proteintech). After incubation with secondary antibodies at room temperature for 30 min and subsequent washes, the bands were visualized using enhanced chemiluminescence (Abcam, USA).
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