Serum and Tissue Biomarker Analysis

The serum levels of D-lactate (DLA) and diamine oxidase (DAO) were quantified with an ELISA kit (Jiangsu Enzyme-Labeled BioTECH, China). Meanwhile, to determine the concentration of IL-1β, IL-6, TNF-α, immunoglobulin A (IgA), and immunoglobulin G (IgG) in the serum, inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in the jejunum and secretory immunoglobulin (SigA) in the colon, ELISA kits (Jiangsu Enzyme-Labeled BioTECH, Yancheng, China) were also used and following the previous study (23 (link)). The protocols were carried out according to the instructions of the manufacturer.

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Jiang Z., Li W., Su W., Wen C., Gong T., Zhang Y., Wang Y., Jin M, & Lu Z. (2021). Protective Effects of Bacillus amyloliquefaciens 40 Against Clostridium perfringens Infection in Mice. Frontiers in Nutrition, 8, 733591.

Publication 2021

Colon Diamine oxidase Elisa Enzyme Il 1β Immunoglobulin Immunoglobulin a Immunoglobulin g Inducible nitric oxide synthase Jejunum Lactate Nitric oxide Secretory Serum Tnf α

Corresponding Organization : Ministry of Agriculture and Rural Affairs

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Serum levels of D-lactate (DLA)
Serum levels of diamine oxidase (DAO)
Serum concentration of IL-1β
Serum concentration of IL-6
Serum concentration of TNF-α
Serum concentration of immunoglobulin A (IgA)
Serum concentration of immunoglobulin G (IgG)
Inducible nitric oxide synthase (iNOS) in the jejunum
Nitric oxide (NO) in the jejunum
Secretory immunoglobulin (SigA) in the colon

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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