Genetic Variants and Diabetes Risk in Women with Previous GDM

DNA was extracted from whole blood using the MaxiPrep Kit (QIAGEN, Sollentuna, Sweden). SNPs were genotyped using a Sequenom massARRAY platform or TaqMan allelic discrimination assay with an ABI Prism 7900 sequence detection system (Applied Biosystems, Foster City, CA, USA). The success rate of genotyping was > 90%. Replication genotyping of 6% of the samples showed > 98% concordance. All SNPs were in Hardy–Weinberg equilibrium (HWE), except for rs11920090 and rs6467136, which significantly deviated in women who did not develop diabetes postpartum (p < 0.01), and were eventually excluded from the analysis. We analysed 12 SNPs previously shown to be associated with measures of insulin secretion [11 (link), 12 (link), 14 (link)], and 4 SNPs previously shown to be associated with measures of insulin resistance in GWAS [10 (link), 11 (link), 13 ], for association with disposition index and HOMA-IR, respectively, in women with previous GDM. We also analysed 70 (2 out of 72 were excluded for not being in HWE) SNPs, previously associated with diabetes in GWAS [11 (link), 15 (link), 18 (link)], for association with diabetes postpartum.

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Prasad R.B., Kristensen K., Katsarou A, & Shaat N. (2021). Association of single nucleotide polymorphisms with insulin secretion, insulin sensitivity, and diabetes in women with a history of gestational diabetes mellitus. BMC Medical Genomics, 14, 274.

Publication 2021

MaxiPrep Kit massARRAY platform ABI Prism 7900 sequence detection system

Allelic Assay Blood Diabetes Discrimination Gwas Insulin resistance Insulin secretion Prism Replication Snps Women

Corresponding Organization :

Other organizations : Lund University

Top 5 similar protocols

Variable analysis

independent variables

SNPs previously shown to be associated with measures of insulin secretion
SNPs previously shown to be associated with measures of insulin resistance in GWAS
SNPs previously associated with diabetes in GWAS

dependent variables

Disposition index
HOMA-IR
Diabetes postpartum

control variables

Genotyping success rate > 90%
Replication genotyping of 6% of the samples showed > 98% concordance
All SNPs were in Hardy–Weinberg equilibrium (HWE), except for rs11920090 and rs6467136, which significantly deviated in women who did not develop diabetes postpartum (p < 0.01) and were eventually excluded from the analysis

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