Human Liver Microsome Preparation

Human microsomes were purchased from Tebu-bio² (Peterborough, UK). The microsomes were prepared from a pool of 50, mixed gender (20 mg protein ml^⁻1) liver samples. DEHTP and MEHTP (purity 98.6%) were provided by BASF SE. All chemicals used were of analytical grade or higher; B-nicotinamide adenine dinucleotide phosphate (NADP), purity 97%, Glucose-6-phosphate, 98%–100%, Magnesium chloride, ACS reagent >99%, and Glucose-6-phosphate dehydrogenase (type V from baker’s yeast) were obtained from Sigma Aldrich. Potassium dihydrogen phosphate, analytical grade, and Di-potassium hydrogen phosphate, analytical grade, were obtained from Fisher Scientific.

Free full text: Click here

McNally K., Sams C., Hogg A, & Loizou G. (2023). Development, testing, parameterisation, and calibration of a human PBPK model for the plasticiser, di-(2-ethylhexyl) terephthalate (DEHTP) using in silico, in vitro and human biomonitoring data. Frontiers in Pharmacology, 14, 1140852.

Publication 2023

B-nicotinamide adenine dinucleotide phosphate (NADP), Glucose-6-phosphate Magnesium chloride Glucose-6-phosphate dehydrogenase Potassium dihydrogen phosphate Di-potassium hydrogen phosphate

Baker yeast Di potassium hydrogen phosphate Glucose 6 phosphate Glucose 6 phosphate dehydrogenase Human Liver Magnesium chloride Microsomes Nicotinamide adenine dinucleotide phosphate Potassium dihydrogen phosphate Protein

Corresponding Organization :

Other organizations : Health & Safety Laboratory

Top 5 similar protocols

Variable analysis

independent variables

DEHTP
MEHTP

dependent variables

Enzyme activity

control variables

Source of human microsomes (pool of 50, mixed gender liver samples)
Protein concentration of microsomes (20 mg protein ml⁻¹)
Purity of DEHTP and MEHTP (98.6%)
NADP (purity 97%)
Glucose-6-phosphate (purity 98%–100%)
Magnesium chloride (purity >99%)
Glucose-6-phosphate dehydrogenase (type V from baker's yeast)
Potassium dihydrogen phosphate (analytical grade)
Di-potassium hydrogen phosphate (analytical grade)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!