Intrahippocampal Infusion of MPEP and CHX

2-methyl-6-phenylethynyl-pyridine(MPEP, CAS: 96206-92-7, Aladdin^®) was dissolved in saline at 0.016 mg/ml. Cannula dummies were removed from guide cannulas and replaced with 26-gauge injectors, which were connected by polyethylene tubing (RWD Life) to 1000-ul microliter syringes mounted in a CMA 402 Microdialysis Syringe Pump. Saline or MPEP was infused 30 min before CFC training at a rate of 0.1 μl/min for 2 min. Cycloheximide (CHX) was dissolved in saline at 50 μg/μl (Quadagno, 1976 (link)). 200 nl solution or saline was injected as described above.

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Xuan S.M., Su Y.W., Liang Y.M., Gao Z.J., Liu C.Y., Fan B.F., Shi Y.W., Wang X.G, & Zhao H. (2023). mGluR5 in amygdala modulates fear memory generalization. Frontiers in Behavioral Neuroscience, 17, 1072642.

Publication 2023

2 methyl 6 phenylethynyl pyridine Cannulas Cycloheximide Microdialysis Polyethylene Saline Syringe

Corresponding Organization : Sun Yat-sen University

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Variable analysis

independent variables

MPEP (2-methyl-6-phenylethynyl-pyridine, CAS: 96206-92-7, Aladdin®) concentration in saline
Cycloheximide (CHX) concentration in saline

dependent variables

Contextual fear conditioning (CFC) training

control variables

Saline solution
Guide cannulas and 26-gauge injectors connected by polyethylene tubing to microliter syringes mounted in a CMA 402 Microdialysis Syringe Pump
Infusion rate of 0.1 μl/min for 2 min

controls

Saline as a negative control for MPEP and CHX

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