All chemicals were purchased from either Sigma-Aldrich or Fisher Scientific. ACS grade methanol, ethyl acetate, toluene, anhydrous DMF, DCM, and DMSO were purchased from Fisher Scientific or Sigma-Aldrich. All reactions were performed under an argon atmosphere. NMR data were collected on a Bruker AVANCE 500 MHz spectrometer, and analysis was carried out using MestReNova. HRMS data was obtained at the Mass Spectrometry Facility at the University of Connecticut. FT-IR analysis was carried out on a Bruker Alpha Platinum ATR instrument using OPUS software (v7.2). The preparation of previously characterized ITZ intermediates (3 -5 ) followed known procedures with minor modifications.11 (link),12 (link),20 (link),21 All ITZ analogues evaluated in the biological assays were greater than 95% pure based on the HPLC methods described below.
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Synthesis and Characterization of ITZ Analogues
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ACS grade methanol
ethyl acetate
toluene
anhydrous DMF
DMSO
AVANCE 500 MHz spectrometer
MestReNova
Alpha Platinum ATR
Argon
Atmosphere
Biological assays
Dmso
Ethyl acetate
Hplc
Mass spectrometry
Methanol
Platinum
Toluene
Corresponding Organization : University of Connecticut
Other organizations : Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute
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- Chemicals used (ACS grade methanol, ethyl acetate, toluene, anhydrous DMF, DCM, and DMSO)
- NMR data
- HRMS data
- FT-IR analysis
- Reactions performed under an argon atmosphere
- Previously characterized ITZ intermediates (
3 -5 ) followed known procedures with minor modifications - All ITZ analogues evaluated in the biological assays were greater than 95% pure based on the HPLC methods described
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