To visualize the viable and dead cells, we used the Live/Dead BacLight Viability kit L13152 (Invitrogen, Molecular Probes, Inc., Eugene, OR, USA). Control and treated cells were stained for 20 min with a 6.0 μM SYTO 9 and 30 μM KI mixture. Fluorescence microscopic imaging of the cells was performed using a LEICA DM 4000 B (Leica Microsystems, Denmark) [20 (link)].
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