Rabbit immune sera raised against DHG from E. faecalis type 2 (α-DHG), SagA (α-SagA), and PpiC (α-PpiC) have been previously described [8 (link), 11 (link), 12 (link)]. For DHG-PpiC and DHG-SagA, New Zealand white rabbits were vaccinated with 2 subcutaneous injections of 10 µg of conjugate in incomplete Freund’s adjuvant given 2 weeks apart. An intravenous injection of 5 µg of the conjugate was given 7 days later, followed by 2 more intravenous injections of 5 µg delivered 2 days apart from each other. On day 35, a test serum was collected, and 7 and 14 days later intravenous boosts with 5 µg of antigen were administered. On day 56, a terminal immune serum was collected from each rabbit. All sera were heat inactivated at 56°C for 30 minutes. Terminal immune sera raised against the conjugates were designated α-DHG-PpiC and α-DHG-SagA. The antibodies were purified using an rProtein A GraviTrap column (GE Healthcare) in accordance with the manufacturers’ instructions. Immunoglobulin G (IgG) contents were measured by enzyme-linked immunosorbent assays (ELISAs) in purified and unpurified sera (Supplementary Materials).