Rabbit Antibodies Production and Purification
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Corresponding Organization : Ludwig-Maximilians-Universität München
Other organizations : Università Cattolica del Sacro Cuore, Agostino Gemelli University Polyclinic, Istituti di Ricovero e Cura a Carattere Scientifico, Universidade da Coruña
Variable analysis
- Vaccination with 2 subcutaneous injections of 10 µg of conjugate in incomplete Freund's adjuvant given 2 weeks apart
- Intravenous injection of 5 µg of the conjugate given 7 days later
- 2 more intravenous injections of 5 µg delivered 2 days apart from each other
- Intravenous boosts with 5 µg of antigen administered 7 and 14 days after the test serum collection
- Anti-DHG (α-DHG), anti-SagA (α-SagA), and anti-PpiC (α-PpiC) antibody levels in the test and terminal immune sera
- Anti-DHG-PpiC (α-DHG-PpiC) and anti-DHG-SagA (α-DHG-SagA) antibody levels in the terminal immune sera
- Rabbit immune sera used
- Heat inactivation of all sera at 56°C for 30 minutes
- Purification of antibodies using an rProtein A GraviTrap column (GE Healthcare)
- Measurement of immunoglobulin G (IgG) contents by enzyme-linked immunosorbent assays (ELISAs) in purified and unpurified sera
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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