Western Blot Antibody Analysis

Western immunoblotting was accomplished as previously described (25 (link),26 (link)) using the following primary antibodies: anti-caspase-9 (Assay Designs); anti-FLAG and anti-β-actin (Sigma); anti-p65, anti-NF-κB2, anti-IκBα, phospho-IκBα, anti-laminA/C, anti-α-tubulin, anti-NIK, anti-RIP1, anti-cIAP1, anti-cIAP2, anti-Apaf1, anti-myc (Cell Signaling Technology); anti-K48, anti-Ki-67, and K63-linkage specific ubiquitin, and anti-Ki-67 (Abcam). Secondary antibodies were horseradish peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG antibodies (Cell Signaling Technology).

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Vu N.T., Park M.A., Shultz M.D., Bulut G.B., Ladd A.C, & Chalfant C.E. (2016). Caspase-9b interacts directly with cIAP1 to drive agonist-independent activation of NF-κB and lung tumorigenesis. Cancer research, 76(10), 2977-2989.

Publication 2016

anti-FLAG and anti-β-actin anti-p65 anti-IκBα phospho-IκBα anti-laminA/C anti-α-tubulin anti-NIK anti-RIP1 anti-cIAP1 anti-cIAP2 anti-Apaf1 anti-myc anti-Ki-67 horseradish peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG antibodies

Actin Anti igg Antibodies Apaf1 Assay Caspase 9 Ciap1 Ciap2 Horseradish peroxidase Mouse Phospho Rabbit Ubiquitin α tubulin

Corresponding Organization : Hunter Holmes McGuire VA Medical Center

Other organizations : Education & Research Foundation

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Protocol cited in 3 other protocols

Variable analysis

independent variables

Anti-caspase-9
Anti-FLAG
Anti-β-actin
Anti-p65
Anti-NF-κB2
Anti-IκBα
Phospho-IκBα
Anti-laminA/C
Anti-α-tubulin
Anti-NIK
Anti-RIP1
Anti-cIAP1
Anti-cIAP2
Anti-Apaf1
Anti-myc
Anti-K48
Anti-Ki-67
K63-linkage specific ubiquitin
Anti-Ki-67

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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