The following procedure was followed for detection. In nuclease assay buffer, 1 μL of RNase inhibitor (New England Biolabs, Inc.), 1 μL LwCas13a, 1.5 μL crRNA, 2 μL NTP Mix (New England Biolabs, Inc.), 0.5 μL of T7 RNA Polymerase (New England Biolabs, Inc.), 0.5 μL of HEPES buffer solution (ThermoFisher Scientific, Inc., USA), 0.25 μL MgCl2 solution (1 M), 2.5 μL quenched fluorescence RNA reporter (RNAse Alert, Thermo Scientific, Waltham, MA, USA), 5 μL of target nucleic acid, and 10.75 μL of RNase-free water were added. The reactions were carried out at 37°C for 1 h, and the fluorescence signal was collected every 2 min on an ABI 7500 Fast DX (Applied Biosystems, Foster City, CA, USA).